A genome-wide association study in 574 schizophrenia trios using DNA pooling

G. Kirov, et al., A genome-wide association study in 574 schizophrenia trios using DNA pooling, Molecular Psychiatry 14, 2009

The cost of genome-wide association (GWA) studies can be prohibitively high when large samples are genotyped. We conducted a GWA study on schizophrenia (SZ) and to reduce the cost, we used DNA pooling. We used a parent-offspring trios design to avoid the potential problems of population stratification. We constructed pools from 605 unaffected controls, 574 SZ patients and a third pool from all the parents of the patients. We hybridized each pool eight times on Illumina HumanHap550 arrays.

We genotyped 63 SNPs in 574 trios and analyses the results with the transmission disequilibrium test. Forty of those were significant at P<0.05.

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