Estimating telomere length from whole genome sequence data

Zhihao Ding, et al., Estimating telomere length from whole genome sequence data, Nucleic Acids Research 42(9), 2014

Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data.

 

We defined reads as telomeric if they contained k or more TTAGGG repeats, with a default threshold value of k = 7.

 

However, in practice with current technology, a typical exome sequencing output contains some fraction (typically 10-50%) of sequence that is off-target, i.e. not exonic. This fraction represents information on the rest of the genome and can be used to estimate relative telomere length by our method.

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