Lars Feuerbach, et al., TelomereHunter: telomere content estimation and characterization from whole genome sequencing data, bioRxiv, 2016
Here, we present TelomereHunter, a new computational tool for determining telomere content that is specifically designed for matched tumor and control pairs. In contrast to existing tools, TelomereHunter takes alignment information into account and reports the abundance of variant repeats in telomeric sequences.
The criterion of searching for six non-consecutive repeats in 100bp long reads has been proposed previously (Lee, et al., 2014) and was also found suitable for the data presented in the present study.
Zhihao Ding, et al., Estimating telomere length from whole genome sequence data, Nucleic Acids Research 42(9), 2014
Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data.
We defined reads as telomeric if they contained k or more TTAGGG repeats, with a default threshold value of k = 7.
However, in practice with current technology, a typical exome sequencing output contains some fraction (typically 10-50%) of sequence that is off-target, i.e. not exonic. This fraction represents information on the rest of the genome and can be used to estimate relative telomere length by our method.
Hilde Nybom, et al., DNA fingerprinting in botany: past, present, future, Investigative Genetics 5:1, 2014
In the 1980s, the REFP methodology was also first applied to the chloroplast DNA (cpDNA) molecule.
Since the cp DNA molecule is highly conserved, there is very little intra-specific variation, and cpDNA-based RELP studies have therefore mostly been conducted on an inter-specific level.
While the plant mitochondrial DNA (mtDNA) sequence is usually highly conserved, the size and structure of mtDNA molecules may vary widely even within individual plants. Moreover, recent studies indicated that substitution rates of mtDNA genes can vary enormously even among closely related plant species.
A special situation is encountered when products from protected trees are involved since woody tissues usually yields heavily degraded DNA. Nevertheless, a set of SNP markers derived from cpDNA intergenic spacers have proven useful for identification of tropical tree species using wood-derived DNA samples.
Sarah Handler, Ming Furniture in the Light of Chinese Architecture, Ten Speed Press, 2005
Most large beds have a canopy, which approximates the walls and roof of a house. Thus, a house within a house. The difference between them is not only size, but also function. The actual house has a floor to stand on. The house within the house was for sitting, sleeping, or making love and conceiving sons. Chinese beds are used for seating during the day and for sleeping at night.